Josephine Mitchell (born 21 May 1965) is an Australian actress and playwright with a lengthy career in theatre and television soap operas and serials.

Jo Mitchell is married to Australian director and producer Chris Martin-Jones and they have two daughters. The pair met when Martin-Jones was a director on A Country Practice in 1989.

She has a double degree from Sydney University, in Medieval and Religious Studies. She is currently focusing on her work behind the camera as writer, having finished a feature-length movie titled The Cult and the TV movie, Aren't U Spesh!